Coding

Part:BBa_K3590012:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_Brno_Czech_Republic   (2020-09-01)


Myc-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 24
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence of Myc-tag (EQKLISEEDL) was rewritten into a nucleotide sequence and codon-optimized for Bacillus subtilis.


Source

The amino acid sequence of Myc-tag (EQKLISEEDL) was rewritten into a nucleotide sequence and codon-optimized for Bacillus subtilis.

References